AtGRP7 qPCR #2

Setup the plate for qPCR of the previous samples, AtGRP7 stuff only.

Mastermix, one for each of 9 samples:
105 2x Bio-rad Sybr
18 water
3 cDNA

Primer mastermix:
66 primer (At3g25470, AtGRP7_spliced, AtGRP7_mature)
198 water

*** Just realized I didn’t add the mature splice primers for the 12hpi hrcC sample. Damn it! ***

.pcrd                PDF

All:


5hpi, 7hpi, 12hpi:


5hpi, 7hpi, 12hpi using the mock as a control:


5hpi and 7hpi using mock as control and Mature as reference (instead of At3g25470)


At first glance, everything is the same as last time we used mature as the reference, except for the 7hpi hrcC. This time shows a drop in the spliced versus the mature at 7hpi, but before it didn’t drop at all, and stayed about a 1:1 ratio the entire time. Last time’s 5hpi and 7hpi (mock isn’t shown, but is set to 1):


Have to re-do the 12hpi stuff... found here