2010-12-27

qPCR - 12hpi and UPF PR-1 stuff

Started qPCR for the UPF PR-1 stuff, as well as the AtGRP7 stuff for the 12hpi, since I forgot to add some primers last time.

2ul cDNA for each mastermix.

Everything: .pcrd        PDF
UPF PDF .xls
AtGRP7 PDF .xls

UPF PR-1:
UPF-2010-12-27-09-58.png
Looks like PR-1 isn’t really induced in the UPF mutants. I’m not sure why I saw something in the Col-0 though.

AtGRP7 with Mature as reference and At3g25470 as reference:
12hpi_mature_ref-2010-12-27-09-58.png 12hpi-2010-12-27-09-58.png

Combining the data from this run and the last run for 5 & 7 hpi to make a new master file. Analysis here.

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