Lab Notebook test
2010-12-23
RNA isolation, day 2 - bioanalyzer, cDNA prep
Submitted the RNA to bioanalyzer. I used 2ul DEPC water and 2ul of the RNA for dilutions.
Order #
1191
Everything looks good.
12/23/10 12:39 PM
Started cDNA using the iScript reaction kit.
3ul RNA
4ul 5x mix
1ul reverse transcriptase
12 water (from the kit)
qPCRs are going to be in there own separate entries:
AtGRP7 5&7hpi 12hpi
UPF
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