2010-12-23

RNA isolation, day 2 - bioanalyzer, cDNA prep

Submitted the RNA to bioanalyzer. I used 2ul DEPC water and 2ul of the RNA for dilutions.

Order # 1191

Everything looks good.

B1191_Kimbrel_2010-12-23_10-19-58_GEL-2010-12-23-09-15.jpg

12/23/10 12:39 PM
Started cDNA using the iScript reaction kit.

3ul RNA
4ul 5x mix
1ul reverse transcriptase
12 water (from the kit)

PastedGraphic10-2010-12-23-09-15.jpg

qPCRs are going to be in there own separate entries:
AtGRP7 5&7hpi        12hpi
UPF

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